Challenge *cough* accepted.

I passed on turbo mode with Lori, didn't die once, nor use any save files. That to be said, Lori is a godsend in 5.0 turbo mode, where you can't spam the shoot button to shoot faster anymore. However, with Jazz (he's the hardest to use in 5.0 turbo mode) I did die once, though I could probably pass without dying. I relied a lot on toaster with Jazz.

I also started right at the diamondus level, so no powerups or ammo.

It took me about 15-20 minutes to do in total.